2008 The Open Forensic Science Journal: Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source: Difference between revisions
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Source: ''The Open Forensic Science Journal'' 2008, Vol. 1, pp. 12-15, DOI: 10.13140/2.1.2897.1848<br> | |||
Source: The Open Forensic Science Journal 2008 | |||
==<font color="orange">Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source</font>== | ==<font color="orange">Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source</font>== | ||
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Authors: A. Fiedler<SUP> 1</SUP>, J. Rehdorf<SUP> 2</SUP>, F. Hilbers<SUP> 3</SUP>, L. Johrdan<SUP> 4</SUP>, J. Rehdorf<SUP> 5</SUP> and M. Benecke<SUP> 6</SUP><br> | Authors: A. Fiedler<SUP> 1</SUP>, J. Rehdorf<SUP> 2</SUP>, F. Hilbers<SUP> 3</SUP>, L. Johrdan<SUP> 4</SUP>, J. Rehdorf<SUP> 5</SUP> and M. Benecke<SUP> 6</SUP><br> | ||
<sup>1</SUP> Paul Flechsig Institute, University of Leipzig, Germany<br> | |||
<sup>1</SUP> Institute for Chemistry and Biochemistry, University of Greifswald, Germany<br> | |||
<sup>1</SUP> University of Osnabrück, Germany<br> | |||
<sup>1</SUP> University of Bochum, Germany<br> | |||
<sup>1</SUP> University of Regensburg, Germany <br> | |||
<sup>1</SUP> Internat. Forensic Research & Consulting, Postfach 250411, 50520 Cologne, Cologne, Germany<br> | |||
Get the complete article here (.PDF): [http://wiki2.benecke.com/images/e/e7/Semen_saliva_UV_mark_benecke_et_al.pdf Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source]<br><br> | |||
[ | Detection of Semen - the poster (.PDF): [http://wiki2.benecke.com/images/9/93/Detection_of_Saliva_and_Semen_with_UV.pdf Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source]<br><br> | ||
[ | [MBs [[All Mark Benecke Publications|publications]]] [More [http://wiki2.benecke.com/index.php?title=All_Mark_Benecke_Publications#Real_Cases cases]] | ||
'' | <font color=orange>'''Abstract'''</font><br> | ||
A possible method to detect body fluids in forensic cases of sexual assault or abuse is by using forensic light sources, which emit certain wavelengths and excite body fluids to fluoresce. However, the success of the visualization of the fluorescence signal can be significantly reduced when unfavorable conditions - especially daylight - are present or used.<br> | |||
We studied the detection of fresh semen (human, boar) and saliva (human) on different and colored types of fabric. The stained samples were stored for 3 and 5 weeks, respectively, and some were additionally washed with detergent at 30°C (86°F). The portable forensic light source Lumatec Superlight 400 which emits wavelengths from 320 nm (UV) to 700 nm (visible light, VIS) and colored goggles and filters were used.<br> | |||
The very high intensity light source detected semen and saliva in darkness and daylight. No difference were found in samples stored 3 and 5 weeks, respectively. Best results for semen and saliva were obtained using wavelengths between 415-490 nm. For general search for body fluids, excitation of 350-500 nm is preferable. With appropriate goggles (orange (>500 nm) or red (>590)) semen could be detected in 100% and saliva in 60% of the cases. Washing the samples with de- tergent removed the biological stains in 75% of the cases. The darker the color, the more the fluorescence signal was ab- sorbed. The type of the fabric had however no significant effect on detection of semen and saliva. The recognition rate of saliva was much better than reported for other light sources.<br> | |||
An unexpected observation on the side was that the fluorescence signal of boar semen was clearly weaker than that of human semen, although the amount of sperm cells per ml is 50-150 million for humans and 25-300 million for boar.<br> | |||
Get the whole article here (.PDF): [http://wiki2.benecke.com/images/e/e7/Semen_saliva_UV_mark_benecke_et_al.pdf Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source]<br><br> | |||
Detection of Semen - the poster (.PDF): [http://wiki2.benecke.com/images/9/93/Detection_of_Saliva_and_Semen_with_UV.pdf Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source]<br><br> | |||
===<font color=orange>Suggested readings</font>=== | |||
<i> | |||
* [[2004 Aggrawals Internet Journal of Forensic Medicine and Toxicology|"I don't know" can be the best answer]]<br> | |||
* [[2012 08 Leica Unity: Looking for the Killer|Looking for the Killer]] | |||
* | * [[2002 Entomology - Insect and Corpses|Entomology - Insects and Corpses]]<br> | ||
* [[2002 Juridical Aspects of the Trials against Serial Killer Juergen Bartsch|Juridical Aspects of the Trials against Serial Killer Juergen Bartsch]]<br> | |||
* [[2006 Forensic biology in the courtroom|Forensic biology in the courtroom]]<br> | |||
* [[2017 09 Reflections of Darkness Artists and their Tattoos Forensic Biologist Dr Mark Benecke|Artists and their Tattoos Forensic Biologist Dr Mark Benecke]]<br> | |||
* [[Bonacci 2017 Flies and ants: A forensic entomological neglect case of an elderly man in Calabria, Southern Italy|Flies and ants: A forensic entomological neglect case of an elderly man in Calabria, Southern Italy]]<br> | |||
* [[2004 Aggrawals Internet Journal of Forensic Medicine and Toxicology Publication Experiment|Aggrawals Internet Journal of Forensic Medicine and Toxicology Publication Experiment]]<br><br> | |||
</i> | |||
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Latest revision as of 15:37, 5 February 2018
Source: The Open Forensic Science Journal 2008, Vol. 1, pp. 12-15, DOI: 10.13140/2.1.2897.1848
Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source
Authors: A. Fiedler 1, J. Rehdorf 2, F. Hilbers 3, L. Johrdan 4, J. Rehdorf 5 and M. Benecke 6
1 Paul Flechsig Institute, University of Leipzig, Germany
1 Institute for Chemistry and Biochemistry, University of Greifswald, Germany
1 University of Osnabrück, Germany
1 University of Bochum, Germany
1 University of Regensburg, Germany
1 Internat. Forensic Research & Consulting, Postfach 250411, 50520 Cologne, Cologne, Germany
Get the complete article here (.PDF): Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source
Detection of Semen - the poster (.PDF): Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source
[MBs publications] [More cases]
Abstract
A possible method to detect body fluids in forensic cases of sexual assault or abuse is by using forensic light sources, which emit certain wavelengths and excite body fluids to fluoresce. However, the success of the visualization of the fluorescence signal can be significantly reduced when unfavorable conditions - especially daylight - are present or used.
We studied the detection of fresh semen (human, boar) and saliva (human) on different and colored types of fabric. The stained samples were stored for 3 and 5 weeks, respectively, and some were additionally washed with detergent at 30°C (86°F). The portable forensic light source Lumatec Superlight 400 which emits wavelengths from 320 nm (UV) to 700 nm (visible light, VIS) and colored goggles and filters were used.
The very high intensity light source detected semen and saliva in darkness and daylight. No difference were found in samples stored 3 and 5 weeks, respectively. Best results for semen and saliva were obtained using wavelengths between 415-490 nm. For general search for body fluids, excitation of 350-500 nm is preferable. With appropriate goggles (orange (>500 nm) or red (>590)) semen could be detected in 100% and saliva in 60% of the cases. Washing the samples with de- tergent removed the biological stains in 75% of the cases. The darker the color, the more the fluorescence signal was ab- sorbed. The type of the fabric had however no significant effect on detection of semen and saliva. The recognition rate of saliva was much better than reported for other light sources.
An unexpected observation on the side was that the fluorescence signal of boar semen was clearly weaker than that of human semen, although the amount of sperm cells per ml is 50-150 million for humans and 25-300 million for boar.
Get the whole article here (.PDF): Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source
Detection of Semen - the poster (.PDF): Detection of Semen (Human and Boar) and Saliva on Fabrics by a Very High Powered UV-/VIS-Light Source
Suggested readings
Dr. rer. medic. Mark Benecke · Diplombiologe (verliehen in Deutschland) · Öffentlich bestellter und vereidigter Sachverständiger für kriminaltechnische Sicherung, Untersuchung u. Auswertung von biologischen Spuren (IHK Köln) · Landsberg-Str. 16, 50678 Köln, Deutschland, E-Mail: forensic@benecke.com · www.benecke.com · Umsatzsteueridentifikationsnummer: ID: DE212749258 · Aufsichtsbehörde: Industrie- und Handelskammer zu Köln, Unter Sachsenhausen 10-26, 50667 Köln, Deutschland · Fallbearbeitung und Termine nur auf echtem Papier. Absprachen per E-mail sind nur vorläufige Gedanken und nicht bindend. 🗺 Dr. Mark Benecke, M. Sc., Ph.D. · Certified & Sworn In Forensic Biologist · International Forensic Research & Consulting · Postfach 250411 · 50520 Cologne · Germany · Text SMS in criminalistic emergencies (never call me): +49.171.177.1273 · Anonymous calls & suppressed numbers will never be answered. · Dies ist eine Notfall-Nummer für SMS in aktuellen, kriminalistischen Notfällen). · Rufen Sie niemals an. · If it is not an actual emergency, send an e-mail. · If it is an actual emergency, send a text message (SMS) · Never call. · Facebook Fan Site · Benecke Homepage · Instagram Fan Page · Datenschutz-Erklärung · Impressum · Archive Page · Kein Kontakt über soziale Netzwerke. · Never contact me via social networks since I never read messages & comments there.